Summary: Journal of Fish Biology (2002) 61, 13091313
doi:10.1006/jfbi.2002.2139, available online at http://www.idealibrary.com on
Genetic tagging to determine passive integrated transponder
tag loss in lemon sharks
K. A. FELDHEIM*§, S. H. GRUBER, J. R. C. DE MARIGNAC AND
M. V. ASHLEY*
*Department of Biological Sciences, University of Illinois at Chicago, 845 West Taylor
Street, Chicago, Illinois 60608, U.S.A., Division of Marine Biology and Fisheries,
Rosenstiel School of Marine and Atmospheric Science, 4600 Rickenbacker Causeway,
Miami, Florida 33149, U.S.A. and Tuna Research and Conservation Center,
Monterey Bay Aquarium, 886 Cannery Row, Monterey, California 93940, U.S.A.
(Received 9 April 2002, Accepted 30 September 2002)
Multilocus genotypes using nine DNA microsatellite loci provided an effective and permanent
individual identification mark for lemon sharks Negaprion brevirostris, the first example of
genetic tagging for any elasmobranch species. The double-tagging provided by micro-
satellites afforded a means to determine passive integrated transponder (PIT) tag shed rate in
lemon sharks. Of 388 sharks that were recaptured, 47 (12·1%) had shed their PIT tag.
2002 The Fisheries Society of the British Isles. Published by Elsevier Science Ltd. All rights reserved.
Key words: passive integrated transponder; microsatellites; Negaprion brevirostris; genetic