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Summary: 1/4 CBEB 2008
CITOMETRIA ÓPTICO-MAGNÉTICA DE ROTAÇÃO: EXEMPLO DE UM
MÉTODO PARA MEDIR AS PROPRIEDADES REOLÓGICAS DA CÉLULA
H. T. Moriya*, D. J. Colen**, G. N. Maksym** e A. M. Alencar***
*Lab.de Engenharia Biomédica/Escola Politécnica da Universidade de São Paulo, São Paulo, Brasil
**Departament of Biomedical Engineering/Dalhousie University, Halifax, Canadá
***Depto de Patologia/Faculdade de Medicina da Universidade de São Paulo, São Paulo, Brasil
e-mail: htmoriya@leb.usp.br
Abstract: The ability for a cell to remodel and
change its cytoskeleton is essential for smooth muscle
function. The Optical Magnetic Twisting Cytometry
(OMTC) is a new method that has been used to study
the cytoskeleton mechanics. We applied the OMTC
technique to characterize the rheology of a sample of
human airway smooth muscle cells. An oscilating
magnetic field of 0.5 Hz were applied before and
after KCl application to the sample. Before
constriction, we measured 11.054.0)´( ±=G Pa/nm
and 05.019.0)´´( ±=G . After KCl application,
)´(G increased to 17.069.0 ± Pa/nm and )´´(G
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