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A nonlinear approach to tracking single nanometer-scale fluorescent Sean B. Andersson

Summary: A nonlinear approach to tracking single nanometer-scale fluorescent
Sean B. Andersson
Department of Mechanical Engineering, Boston University, Boston, MA 02215
Abstract-- We describe a nonlinear control law for tracking
single, sub-diffraction limit fluorescent particles that takes
advantage of the geometric properties of the spatial intensity
pattern. We present simulation results for tracking a single
particle based on confocal measurements to illustrate the
approach. The control law converts each intensity measurement
into actuator commands directly, leading to improved temporal
response over existing algorithms. We consider both two- and
three-dimensional tracking.
The ability to image and analyze single molecules is a
powerful tool in molecular biology that continues to be
used to significantly advance knowledge of a wide-range of
systems. There are a wide variety of methods yielding sub-
diffraction limit position accuracy in two and three dimen-


Source: Andersson, Sean B. - Department of Aerospace and Mechanical Engineering, Boston University


Collections: Engineering