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APPLIED AND ENVIRONMENTAL MICROBIOLOGY, Oct. 2002, p. 48204826 Vol. 68, No. 10 0099-2240/02/$04.00 0 DOI: 10.1128/AEM.68.10.48204826.2002
 

Summary: APPLIED AND ENVIRONMENTAL MICROBIOLOGY, Oct. 2002, p. 48204826 Vol. 68, No. 10
0099-2240/02/$04.00 0 DOI: 10.1128/AEM.68.10.48204826.2002
Copyright 2002, American Society for Microbiology. All Rights Reserved.
Sequencing and Transcriptional Analysis of the Chlorite Dismutase
Gene of Dechloromonas agitata and Its Use as a Metabolic Probe
Kelly S. Bender, Susan M. O'Connor, Romy Chakraborty, John D. Coates,
and Laurie A. Achenbach*
Department of Microbiology, Southern Illinois University, Carbondale, Illinois 62901
Received 23 April 2002/Accepted 3 July 2002
The dismutation of chlorite into chloride and O2 represents a central step in the reductive pathway of
perchlorate that is common to all dissimilatory perchlorate-reducing bacteria and is mediated by a single
enzyme, chlorite dismutase. The chlorite dismutase gene cld was isolated and sequenced from the perchlorate-
reducing bacterium Dechloromonas agitata strain CKB. Sequence analysis identified an open reading frame of
834 bp that would encode a mature protein with an N-terminal sequence identical to that of the previously
purified D. agitata chlorite dismutase enzyme. The predicted translation product of the D. agitata cld gene is a
protein of 277 amino acids (aa), including a leader peptide of 26 aa. Primer extension analysis identified a
single transcription start site directly downstream of an AT-rich region that could represent the 10 promoter
region of the D. agitata cld gene. Northern blot analysis indicated that the cld gene was transcriptionally
up-regulated when D. agitata cells were grown in perchlorate-reducing versus aerobic conditions. Slot blot
hybridizations with a D. agitata cld probe demonstrated the conservation of the cld gene among perchlorate-

  

Source: Achenbach, Laurie A. - Department of Microbiology, Southern Illinois University

 

Collections: Environmental Sciences and Ecology; Environmental Management and Restoration Technologies; Biology and Medicine