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An ordered, nonredundant library of Pseudomonas aeruginosa strain PA14 transposon insertion mutants
 

Summary: An ordered, nonredundant library of Pseudomonas
aeruginosa strain PA14 transposon insertion mutants
Nicole T. Liberati*, Jonathan M. Urbach*, Sachiko Miyata, Daniel G. Lee, Eliana Drenkard, Gang Wu, Jacinto Villanueva
,
Tao Wei
, and Frederick M. Ausubel§
Department of Genetics, Harvard Medical School, and Department of Molecular Biology, Massachusetts General Hospital, Boston, MA 02114
Contributed by Frederick M. Ausubel, December 22, 2005
Random transposon insertion libraries have proven invaluable in
studying bacterial genomes. Libraries that approach saturation
must be large, with multiple insertions per gene, making compre-
hensive genome-wide scanning difficult. To facilitate genome-
scale study of the opportunistic human pathogen Pseudomonas
aeruginosa strain PA14, we constructed a nonredundant library of
PA14 transposon mutants (the PA14NR Set) in which nonessential
PA14 genes are represented by a single transposon insertion
chosen from a comprehensive library of insertion mutants. The
parental library of PA14 transposon insertion mutants was gener-
ated by using MAR2xT7, a transposon compatible with transposon-
site hybridization and based on mariner. The transposon-site

  

Source: Ausubel, Frederick M. - Department of Genetics, Harvard University

 

Collections: Biology and Medicine