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A family of thermostable fungal cellulases created by structure-guided recombination
 

Summary: A family of thermostable fungal cellulases created
by structure-guided recombination
Pete Heinzelmana
, Christopher D. Snowa
, Indira Wua
, Catherine Nguyena
, Alan Villalobosb
, Sridhar Govindarajanb
,
Jeremy Minshullb
, and Frances H. Arnolda,1
aDivision of Chemistry and Chemical Engineering 210-41, California Institute of Technology, Pasadena, CA 91125; and bDNA2.0, Menlo Park, CA 94025
Contributed by Frances H. Arnold, February 9, 2009 (sent for review January 19, 2009)
SCHEMA structure-guided recombination of 3 fungal class II cello-
biohydrolases (CBH II cellulases) has yielded a collection of highly
thermostable CBH II chimeras. Twenty-three of 48 genes sampled
from the 6,561 possible chimeric sequences were secreted by the
Saccharomyces cerevisiae heterologous host in catalytically active
form. Five of these chimeras have half-lives of thermal inactivation
at 63 C that are greater than the most stable parent, CBH II enzyme

  

Source: Arnold, Frances H. - Division of Chemistry and Chemical Engineering, California Institute of Technology

 

Collections: Chemistry; Biology and Medicine