Summary: Functional analyses of the »owering time gene OsMADS50,
the putative SUPPRESSOR OF OVEREXPRESSION OF CO 1/
AGAMOUS-LIKE 20 (SOC1/AGL20) ortholog in rice
Shinyoung Lee, Joonyul Kimy
, Jong-Jin Han, Min-Jung Han and Gynheung An├
National Research Laboratory of Plant Functional Genomics, Division of Molecular and Life Sciences, Pohang University
of Science and Technology (POSTECH), Pohang 790-784, Korea
Received 3 December 2003; revised 9 February 2004; accepted 16 February 2004.
For correspondence (fax 82 54 279 0659; e-mail firstname.lastname@example.org).
Present address: MSU-DOE Plant Research Laboratory and Department of Biochemistry and Molecular Biology, Michigan State University, East Lansing,
MI 48824, USA.
A late-»owering mutant was isolated from rice T-DNA-tagging lines. T-DNA had been integrated into the
K-box region of Oryza sativa MADS50 (OsMADS50), which shares 50.6% amino acid identity with the
Arabidopsis MADS-box gene SUPPRESSOR OF OVEREXPRESSION OF CO 1/AGAMOUS-LIKE 20 (SOC1/
AGL20). While overexpression of OsMADS50 caused extremely early »owering at the callus stage,
OsMADS50 RNAi plants exhibited phenotypes of late »owering and an increase in the number of elon-
gated internodes. This con«rmed that the phenotypes observed in the knockout (KO) plants are because of