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Proc. Natl. Acad. Sci. USA Vol. 96, pp. 50495054, April 1999
 

Summary: Proc. Natl. Acad. Sci. USA
Vol. 96, pp. 5049­5054, April 1999
Developmental Biology
Double-stranded RNA specifically disrupts gene expression during
planarian regeneration
ALEJANDRO SA´NCHEZ ALVARADO* AND PHILLIP A. NEWMARK
Carnegie Institution of Washington, Department of Embryology, 115 West University Parkway, Baltimore, MD 21210
Communicated by Donald D. Brown, Carnegie Institution of Washington, Baltimore, MD, February 19, 1999 (received for review January 22, 1999)
ABSTRACT Metazoan regeneration is one of the least
understood fundamental problems of biology. The lack of
progress in understanding this phenomenon at the molecular
level has been due to the poor regenerative abilities of the
genetic organisms used for developmental studies, as well as
the difficulties encountered with molecular and genetic ma-
nipulations of the commonly studied vertebrate models (the
urodele amphibians). Here, we demonstrate that introduction
of double-stranded RNA selectively abrogates gene function in
planarians, a classic model of regeneration. The ability to
eliminate gene function in a regenerating organism such as the
planarian overcomes previous experimental limitations and

  

Source: Alvarado, Alejandro Sánchez - Department of Neurobiology and Anatomy, University of Utah
Newmark, Phillip A. - Department of Cell and Developmental Biology, University of Illinois at Urbana-Champaign

 

Collections: Biology and Medicine