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Cell, Vol. 85, 115124, April 5, 1996, Copyright 1996 by Cell Press RNase III Cleaves Eukaryotic Preribosomal RNA

Summary: Cell, Vol. 85, 115­124, April 5, 1996, Copyright ©1996 by Cell Press
RNase III Cleaves Eukaryotic Preribosomal RNA
at a U3 snoRNP-Dependent Site
Sherif Abou Elela, Haller Igel, (Beltrame and Tollervey, 1995), and U14 base-pairs with
18S sequences (Liang and Fournier, 1995), but neitherand Manuel Ares, Jr.
Center for the Molecular Biology of RNA of these interactions occurs adjacent to a cleavage site.
The requirement for several snoRNAs in pre-18S rRNABiology Department
Sinsheimer Laboratories processing has led to the hypothesis that a multi-snRNP
processing complex akin to the spliceosome is respon-University of California, Santa Cruz
Santa Cruz, California 95064 sible (for reviews see Maxwell and Fournier, 1995; Bach-
ellerie et al., 1995). As more snoRNAs have been identi-
fied, a striking pattern of extensive complementarity with
rRNA has emerged, leading to discussion of the hypoth-Summary
esis that snoRNAs assist correct folding of rRNA (Bach-
ellerie et al., 1995; Steitz and Tycowski, 1995). HowA yeast gene homologous to bacterial RNase III (RNT1)
snoRNAs function in the processing pathway has yet toencodes a double-strand­specific endoribonuclease
be determined.essential for ribosome synthesis. Two rRNA pro-
The biochemical nature of the endonuclease activitiescessing events are blocked in cells temperature sensi-
required for eukaryotic rRNA processing has remainedtive for RNT1: cleavage at the snoRNA-dependent A0
elusive. A crude in vitro system faithfully cleaves verte-site in the 5 ETS and cleavage in the 3 ETS. Recombi-


Source: Abou Elela, Sherif - Département de Microbiologie et d'Infectiologie, Université de Sherbrooke
Ares Jr., Manny - Department of Molecular, Cell, and Developmental Biology, University of California at Santa Cruz


Collections: Biology and Medicine