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Reverse genetic approaches for functional genomics of rice Gynheung An*, Dong-Hoon Jeong, Ki-Hong Jung and Sichul Lee
 

Summary: Reverse genetic approaches for functional genomics of rice
Gynheung An*, Dong-Hoon Jeong, Ki-Hong Jung and Sichul Lee
National Research Laboratory of Plant Functional Genomics, Division of Molecular and Life Sciences,
Pohang University of Science and Technology (POSTECH), Pohang 790-784, Korea (*author for corre-
spondence; e-mail genean@postech.ac.kr)
Received 13 April 2004; accepted in revised form 30 September 2004
Key words: functional genomics, gene tagging, reverse genetics, transposon, T-DNA
Abstract
T-DNA and transposable elements, e.g., Ds and Tos17, are used to generate a large number of insertional
mutant lines in rice. Some carry the GUS or GFP reporter for gene trap or enhancer trap. These reporter
systems are valuable for identifying tissue- or organ-preferential genes. Activation tagging lines have also
been generated for screening mutants and isolating mutagenized genes. To utilize these resources more
efficiently, tagged lines have been produced for reverse genetic approaches. DNA pools of the T-DNA
tagged lines and Tos17 lines have been prepared for PCR screening of insertional mutants in a given gene.
Tag end sequences (TES) of the inserts have also been produced. TES databases are beneficial for analyzing
the function of a large number of rice genes.
Abbreviations: iPCR, inverse PCR; RT-PCR, reverse transcription-PCR; TAIL PCR, thermal asymmetric
interlaced PCR; T-DNA, transferred DNA; UTR, untranslated region
Introduction
Sequencing of the rice genome is nearly complete

  

Source: An, Gynheung - Department of Life Science, Pohang University of Science and Technology

 

Collections: Biotechnology; Biology and Medicine