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Journal of NeuroscienceMethods, 45 (1992)35-40 35 1992Elsevier Science Publishers B.V. All rights reserved 0165-0270/92/$05.00
 

Summary: Journal of NeuroscienceMethods, 45 (1992)35-40 35
1992Elsevier Science Publishers B.V. All rights reserved 0165-0270/92/$05.00
NSM 01416
Biotin-dextran: a sensitive anterograde tracer for neuroanatomic
studies in rat and monkey
Heike M. Brandt and A. Vania Apkarian
Department of Neurosurgery, SUNY Health Science Center, Syracuse,IVY13210 (USA)
(Received 14April 1992)
(Revisedversion received17July1992)
(Accepted 23 July1992)
Key words: Biotin; Dextran; Retrograde label; Axonal label; Diaminobenzidine; Spinal cord
The properties of a new anterograde tracer, biotin-dextran (BD), are reported. Iontophoretic or pressure injectionsof BD in
the spinal cord of rats and monkeysrevealed terminal-likeBD labelin manyregions of the brain. The BD label was visualizedby
an avidin-biotin reaction combined with diaminobenzidine, with and without nickel enhancement. This reaction resulted in
permanent label and revealed the fine morphologyof terminal boutons and en-passant endings. Biotin-dextran is an excellent
anterograde tracer that can also be visualized at the electron microscopiclevel, used in combination with other retrograde and
anterograde tracers and with immunohistochemicallabeling of neurotransmitters.
Introduction
Extracellularly injected anterograde tracers
have been used in the past to study the connectiv-

  

Source: Apkarian, A. Vania - Department of Physiology, Northwestern University

 

Collections: Biology and Medicine