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Volume 15 Number 19 1987 Nucleic Acids Research Deduced prducts of C4-dkarboxylate tranort regultory genes of Rukobium kgminowsaum
 

Summary: Volume 15 Number 19 1987 Nucleic Acids Research
Deduced prducts of C4-dkarboxylate tranort regultory genes of Rukobium kgminowsaum
are homolgous to nirWgen r tory gene produc
Clive W.Ronson123, Patricia M.Astwood1, B.Tracy Nixon24 and Frederick M.Ausubel2
'Grasslands Division, Department of Scientific and hdustrial Research, Private Bag, Palmerston
North, New Zealand and 2Department of Genetics, Harvard Medical School and Department of
Molecular Biology, Massachusetts General Hospital, Boston, MA 02114, USA
Received August 10, 1987; Accepted September 9, 1987
ABSWIACT
We have sequenced two genes dctB and dctD required for the activation of the
C -dicarboxylate transport structural gene dctA in free-living Rhizobium
lgguminosarum. The hydropathic profile of thiedctB gene product (DctB)
suggestedt t its N-terminal region may be lociate in the periplasm and its
C-terminal region in the cytoplasm. The C-terminal region of DctB was
strongly conserved with similar regions of the products of several regulatory
genes that may act as environmental sensors, including ntrB, envZ, virA, p
PxA and p . The N-terminal domain of the dctD gene poduct (DctD)was
strongly conserved with N-terminal domains of theproducts of several
regulatory genes thought to be transcriptional activators, including ntrC,
ompR, virG, p and sfrA. In addition, the central and C-terminal riegons of

  

Source: Ausubel, Frederick M. - Department of Genetics, Harvard University

 

Collections: Biology and Medicine