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MINI-REVIEW Advances in the use of terminal restriction fragment length

Advances in the use of terminal restriction fragment length
polymorphism (T-RFLP) analysis of 16S rRNA
genes to characterize microbial communities
Ursel M. E. Schütte & Zaid Abdo & Stephen J. Bent &
Conrad Shyu & Christopher J. Williams &
Jacob D. Pierson & Larry J. Forney
Received: 20 March 2008 /Revised: 29 May 2008 /Accepted: 1 June 2008
# Springer-Verlag 2008
Abstract Terminal restriction fragment length polymor-
phism (T-RFLP) analysis is a popular high-throughput
fingerprinting technique used to monitor changes in the
structure and composition of microbial communities. This
approach is widely used because it offers a compromise
between the information gained and labor intensity. In this
review, we discuss the progress made in T-RFLP analysis
of 16S rRNA genes and functional genes over the last
10 years and evaluate the performance of this technique
when used in conjunction with different statistical methods.
Web-based tools designed to perform virtual polymerase


Source: Abdo, Zaid - Departments of Mathematics & Statistics, University of Idaho


Collections: Mathematics; Biology and Medicine