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JouirAL OF BACTERIOLOGY, Nov. 1974, p. 815-821 Copyright i) 1974 American Society for Microbiology
 

Summary: JouirAL OF BACTERIOLOGY, Nov. 1974, p. 815-821
Copyright i) 1974 American Society for Microbiology
Vol. 120, No. 2
Printed in U.S.A.
Regulation ofNitrogen Fixation in Klebsiellapneumoniae:
Evidence for a Role of Glutamine Synthetase as a
Regulator of Nitrogenase Synthesis
STANLEY L. STREICHER, K. T. SHANMUGAM, FREDERICK AUSUBEL,
CARLO MORANDL AND RICHARD B. GOLDBERG2
Department ofBiology, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, and
Department of Chemistry, University of California at San Diego, La Jolla California 92037
Received for publication 8 August 1974
Mutations causing constitutive synthesis of glutamine synthetase (GlnC- phe-
notype) were transferred from Klebsiella aerogenes into Klebsiella pneumoniae
by P1-mediated transduction. Such GlnC- strains ofK. pneumoniae have consti-
tutive levels of glutamine synthetase. Two of three GlnC strains of K. pneu-
moniae studied, each containing independently isolated mutations that confer the
GlnC- phenotype, continue to synthesize nitrogenase in the presence of NH4+.
One strain, KP5069, produces 30% as much nitrogenase when grown in the pres-
ence of 15 mM NH4+ as in its absence. The GlnC- phenotype allows the synthe-

  

Source: Ausubel, Frederick M. - Department of Genetics, Harvard University

 

Collections: Biology and Medicine