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Biostatistics (2003), 4, 3, pp. 465477 Printed in Great Britain
 

Summary: Biostatistics (2003), 4, 3, pp. 465477
Printed in Great Britain
The efficiency of pooling mRNA in microarray
experiments
C. M. KENDZIORSKI
Department of Biostatistics and Medical Informatics, University of Wisconsin, 6729 Medical
Sciences Center, 1300 University Avenue, Madison, WI 53792, USA
kendzior@biostat.wisc.edu
Y. ZHANG
Department of Statistics, University of Wisconsin, Madison, WI 53792, USA
H. LAN, A. D. ATTIE
Department of Biochemistry, University of Wisconsin, Madison, WI 53792, USA
SUMMARY
In a microarray experiment, messenger RNA samples are oftentimes pooled across subjects out of
necessity, or in an effort to reduce the effect of biological variation. A basic problem in such experiments
is to estimate the nominal expression levels of a large number of genes. Pooling samples will affect
expression estimation, but the exact effects are not yet known as the approach has not been systematically
studied in this context. We consider how mRNA pooling affects expression estimates by assessing the
finite-sample performance of different estimators for designs with and without pooling. Conditions under
which it is advantageous to pool mRNA are defined; and general properties of estimates from both pooled

  

Source: Attie, Alan D. - Department of Biochemistry, University of Wisconsin at Madison

 

Collections: Biology and Medicine