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T-DNA tagged knockout mutation of rice OsGSK1, an orthologue of Arabidopsis BIN2, with enhanced tolerance to various abiotic
 

Summary: T-DNA tagged knockout mutation of rice OsGSK1, an orthologue
of Arabidopsis BIN2, with enhanced tolerance to various abiotic
stresses
Serry Koh Sang-Choon Lee Min-Kyung Kim Jun Ho Koh
Sichul Lee Gynheung An Sunghwa Choe Seong-Ryong Kim
Received: 5 February 2007 / Accepted: 21 July 2007 / Published online: 10 August 2007
Springer Science+Business Media B.V. 2007
Abstract T-DNA-tagged rice plants were screened
under cold- or salt-stress conditions to determine the
genes involved in the molecular mechanism for their
abiotic-stress response. Line 0-165-65 was identified as
a salt-responsive line. The gene responsible for this GUS-
positive phenotype was revealed by inverse PCR as
OsGSK1 (Oryza sativa glycogen synthase kinase3-like
gene 1), a member of the plant GSK3/SHAGGY-like
protein kinase genes and an orthologue of the Arabidopsis
brassinosteroid insensitive 2 (BIN2), AtSK21. Northern
blot analysis showed that OsGSK1 was most highly
detected in the developing panicles, suggesting that its
expression is developmental stage specific. Knockout

  

Source: An, Gynheung - Department of Life Science, Pohang University of Science and Technology

 

Collections: Biotechnology; Biology and Medicine