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Functional and Structural Analysis of the GABAA Receptor 1 Subunit during Channel Gating and Alcohol Modulation*
 

Summary: Functional and Structural Analysis of the GABAA Receptor 1
Subunit during Channel Gating and Alcohol Modulation*
Received for publication, August 27, 2004, and in revised form, October 18, 2004
Published, JBC Papers in Press, November 2, 2004, DOI 10.1074/jbc.M409871200
Sangwook Jung§, Myles H. Akabas¶, and R. Adron Harris
From the Cell and Molecular Biology Program and Waggoner Center for Alcohol and Addiction Research, University of
Texas at Austin, Austin, Texas 78712 and the ¶Departments of Physiology and Biophysics and of Neuroscience, Albert
Einstein College of Medicine of Yeshiva University, Bronx, New York 10461
The substituted cysteine accessibility method has
proven useful for investigating structural changes of the
-aminobutyric acid type A (GABAA) receptor during
channel gating and allosteric modulation. In the present
study, the surface accessibility and reaction rate of pro-
pyl- and hexyl-methanethiosulfonate to cysteine resi-
dues introduced into the third transmembrane segment
of the GABAA receptor 1 subunit were examined.
GABA-induced currents in Xenopus oocytes expressing
wild type and cysteine mutant GABAA receptors were
recorded before and after application of methanethio-
sulfonate (MTS) reagents in the resting, GABA- or alco-

  

Source: Akabas, Myles - Department of Physiology and Biophysics, Albert Einstein College of Medicine, Yeshiva University

 

Collections: Biology and Medicine