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NMR Detection with Multiple Solenoidal Microcoils for Continuous-Flow Capillary Electrophoresis
 

Summary: NMR Detection with Multiple Solenoidal Microcoils
for Continuous-Flow Capillary Electrophoresis
Andrew M. Wolters, Dimuthu A. Jayawickrama, Andrew G. Webb, and Jonathan V. Sweedler*
Department of Chemistry and the Beckman Institute, and Department of Electrical and Computer Engineering, University of
Illinois, Urbana, Illinois 61801
Nuclear magnetic resonance (NMR) spectroscopy repre-
sents a promising on-line detector for capillary electro-
phoresis (CE). The inherent poor sensitivity of NMR
mandates the use of NMR probes with the highest mass
sensitivity, such as those containing solenoidal microcoils,
for CE/NMR hyphenation. However, electrophoretic cur-
rent degrades the resolution of NMR spectra obtained
from solenoidal coils. A new method to avoid microcoil
NMR spectral degradation during continuous-flow CE is
demonstrated using a unique multiple solenoidal coil
NMR probe. The electrophoretic flow from a single
separation capillary is split into multiple outlets, each
possessing its own NMR detection coil. While the CE
electrophoretic flow is directed through one outlet, stopped-
flow, high-resolution NMR spectra are obtained from the

  

Source: Andrews, Anne M. - Huck Institutes of the Life Sciences, Pennsylvania State University

 

Collections: Biology and Medicine