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Molecular Ecology Notes (2004) 4, 352354 doi: 10.1111/j.1471-8286.2004.00694.x 2004 Blackwell Publishing Ltd
 

Summary: Molecular Ecology Notes (2004) 4, 352354 doi: 10.1111/j.1471-8286.2004.00694.x
2004 Blackwell Publishing Ltd
Blackwell Publishing, Ltd.
PRIMER NOTE
Novel tetranucleotide microsatellite markers from the Del
Norte salamander (Plethodon elongatus) with application
to its sister species the Siskiyou Mountain salamander
(P. stormi)
DOUGLAS J. DEGROSS, LOUISE S. MEAD and STEVAN J. ARNOLD
Department of Zoology, 3029 Cordley Hall, Oregon State University, Corvallis, OR 97331, USA
Abstract
Eleven tetranucleotide microsatellite loci were developed for the Del Norte salamander
(Plethodon elongatus). The loci were variably polymorphic, ranging from two to 20 alleles
per locus, with expected heterozygosities ranging from 0.07 to 0.86. The loci also amplified
in a congener, the Siskiyou Mountain salamander (P. stormi). The microsatellite loci will
be used to assess the utility of highly polymorphic markers to assay within- and between-
species differentiation between these two closely related species.
Keywords: Del Norte salamander, microsatellites, Plethodon, Siskiyou Mountain salamander
Received 19 February 2004; revision accepted 7 April 2004
Plethodontid salamanders are model organisms for address-

  

Source: Arnold, Stevan J. - Department of Zoology, Oregon State University

 

Collections: Biology and Medicine; Environmental Sciences and Ecology