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Distinct microtubule and chromatin characteristics of human oocytes after failed in-vivo and in-vitro meiotic
 

Summary: Distinct microtubule and chromatin characteristics of
human oocytes after failed in-vivo and in-vitro meiotic
maturation
Catherine M.H.Combelles1,3
, David F.Albertini2
and Catherine Racowsky1
1
Department of Obstetrics and Gynecology, Brigham and Women's Hospital, Harvard Medical School, Boston and 2
Department of
Anatomy and Cellular Biology, Tufts University School of Medicine, Boston, Massachusetts, USA
3
To whom correspondence should be addressed at: Department of Obstetrics and Gynecology, Brigham and Women's Hospital,
75 Francis Street, ASB 1+3, Room 082, Boston, MA 02115, USA. E-mail: ccombelles@partners.org
BACKGROUND: While a complete failure of meiotic maturation following hCG administration is rare during IVF
cycles, cases arise in which patients repeatedly display a high incidence of failure to complete maturation to meta-
phase II (MII) in vivo. For the immature oocytes of such patients, our objectives were (i) to ask whether progression
to MII could be supported in vitro, and (ii) to deŽne their microtubule/chromatin properties following in-vitro mat-
uration (IVM). Together, these studies were aimed at augmenting our understanding of factors underlying meiotic
arrest in the human. METHODS: Cases are presented here for two patients (A and B) producing oocytes that recur-
rently showed the inability to mature to metaphase II in vivo. Following IVM attempts, chromatin and microtubule

  

Source: Albertini, David - Center for Reproductive Sciences, University of Kansas

 

Collections: Biology and Medicine