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Molecular Ecology Notes (2003) 3, 253255 doi: 10.1046/j.1471-8286.2003.00415.x 2003 Blackwell Publishing Ltd
 

Summary: Molecular Ecology Notes (2003) 3, 253­255 doi: 10.1046/j.1471-8286.2003.00415.x
© 2003 Blackwell Publishing Ltd
Blackwell Publishing Ltd.
PRIMER NOTE
New microsatellite markers for the endemic Mediterranean
seagrass Posidonia oceanica
F. ALBERTO,* L. CORREIA,* S. ARNAUD-HAOND,* C. BILLOT,* C. M. DUARTE and E. SERRÃO*
*CCMAR -- Center for Marine Sciences, F.C.M.A.- University Algarve, Gambelas, P-8005­139, Faro, Portugal, IMEDEA
(CSIC-UIB), C/Miquel Marques 21, 07190 Esporles, Mallorca, Spain
Abstract
The seagrass Posidonia oceanica is endemic to the Mediterranean Sea, where it plays an
important role in coastal ecosystem dynamics. Because of this important role and concerns
about the observed regression of some meadows, population genetic studies of this species
have been promoted. However, the markers used until now were not polymorphic enough
to efficiently assess the level and spatial pattern of genetic variability. Hypervariable
molecular markers were obtained by screening a genomic library enriched for microsatellite
dinucleotide repeats. Among 25 primer pairs defined, eight amplified polymorphic micro-
satellites with an encouraging level of variability at the two geographical scales sampled.
Keywords: clonal plant, dinucleotides, microsatellites, Posidoniaceae, seagrass
Received 4 December 2002; revision accepted 17 January 2003

  

Source: Alberto, Filipe - Centro de Ciencias do Mar, Universidade do Algarve

 

Collections: Environmental Sciences and Ecology; Biology and Medicine