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TECHNICAL NOTE Development and characterization of highly polymorphic

Development and characterization of highly polymorphic
microsatellite loci for the Western Spadefoot toad,
Pelobates cultripes
Mirjam S. van de Vliet Onno E. Diekmann
Ester T. A. Serra~o Pedro Beja
Received: 11 July 2008 / Accepted: 13 August 2008 / Published online: 29 August 2008
Springer Science+Business Media B.V. 2008
Abstract Nine highly polymorphic microsatellite markers
were isolated and characterized for the Western Spadefoot,
Pelobates cultripes. Remarkably, for this amphibian species
high numbers of microsatellites were found as part of larger
repeat containing regions, making primer design difficult.
For nine loci, primers were designed successfully and
genotyping of individuals was reliable and consistent.
Number of alleles and heterozygosity for these loci ranged
from 9 to 34 and from 0.72 to 0.94, respectively. The high
levels of polymorphism revealed by our developed loci
should provide insight into population genetic structure and
levels of dispersal for this typical Mediterranean temporary


Source: Alberto, Filipe - Centro de Ciencias do Mar, Universidade do Algarve


Collections: Environmental Sciences and Ecology; Biology and Medicine