Home

About

Advanced Search

Browse by Discipline

Scientific Societies

E-print Alerts

Add E-prints

E-print Network
FAQHELPSITE MAPCONTACT US


  Advanced Search  

 
TECHNICAL NOTE Development and characterization of highly polymorphic
 

Summary: TECHNICAL NOTE
Development and characterization of highly polymorphic
microsatellite loci for the Western Spadefoot toad,
Pelobates cultripes
Mirjam S. van de Vliet Onno E. Diekmann
Ester T. A. Serra~o Pedro Beja
Received: 11 July 2008 / Accepted: 13 August 2008 / Published online: 29 August 2008
Springer Science+Business Media B.V. 2008
Abstract Nine highly polymorphic microsatellite markers
were isolated and characterized for the Western Spadefoot,
Pelobates cultripes. Remarkably, for this amphibian species
high numbers of microsatellites were found as part of larger
repeat containing regions, making primer design difficult.
For nine loci, primers were designed successfully and
genotyping of individuals was reliable and consistent.
Number of alleles and heterozygosity for these loci ranged
from 9 to 34 and from 0.72 to 0.94, respectively. The high
levels of polymorphism revealed by our developed loci
should provide insight into population genetic structure and
levels of dispersal for this typical Mediterranean temporary

  

Source: Alberto, Filipe - Centro de Ciencias do Mar, Universidade do Algarve

 

Collections: Environmental Sciences and Ecology; Biology and Medicine