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The Yeast Fre1p/Fre2p Cupric Reductases Facilitate Copper Uptake and Are Regulated by the Copper-modulated Mac1p Activator*
 

Summary: The Yeast Fre1p/Fre2p Cupric Reductases Facilitate Copper Uptake
and Are Regulated by the Copper-modulated Mac1p Activator*
(Received for publication, July 18, 1996, and in revised form, February 11, 1997)
Elena Georgatsou, Lampros A. Mavrogiannis§, George S. Fragiadakis§,
and Despina Alexandraki§¶
From the Foundation for Research and Technology-Hellas, Institute of Molecular Biology and Biotechnology,
and Department of Biology, University of Crete, Heraklion 711 10 Crete, Greece
Fre1p and Fre2p are ferric reductases which account
for the total plasma membrane associated activity, a
prerequisite for iron uptake, in Saccharomyces cerevi-
siae. The two genes are transcriptionally induced by
iron depletion. In this communication, we provide evi-
dence that Fre2p has also cupric reductase activity, as
has been previously shown for Fre1p (Hassett, R., and
Kosman, D.J. (1995) J. Biol. Chem. 270, 128­134). Both
Fre1p and Fre2p enzymes are functionally significant
for copper uptake, as monitored by the accumulation of
the copper-regulated CUP1 and CTR1 mRNAs in fre1 ,
fre2 , and fre1 fre2 mutant strains. However, only
Fre1p activity is induced by copper depletion, even in

  

Source: Alexandraki, Despina - Institute of Molecular Biology and Biotechnology, Foundation of Research and Technology, Hellas

 

Collections: Biology and Medicine; Biotechnology