Home

About

Advanced Search

Browse by Discipline

Scientific Societies

E-print Alerts

Add E-prints

E-print Network
FAQHELPSITE MAPCONTACT US


  Advanced Search  

 
Systematic functional analysis of the Caenorhabditis elegans
 

Summary: Systematic functional analysis
of the Caenorhabditis elegans
genome using RNAi
Ravi S. Kamath*, Andrew G. Fraser*§, Yan Dong*, Gino Poulin*, Richard Durbin, Monica Gotta*§, Alexander Kanapink, Nathalie Le Bot*,
Sergio Moreno*{, Marc Sohrmann§, David P. Welchman*, Peder Zipperlen* & Julie Ahringer*
* Wellcome Trust/Cancer Research UK Institute and Department of Genetics, University of Cambridge, Tennis Court Road, Cambridge CB2 1QR, UK
Wellcome Trust Sanger Institute, Hinxton, Cambridge CB10 1SA, UK
k EMBL-European Bioinformatics Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge CB10 1SD, UK
{ Centro de Investigacion del Cancer, CSIC / Univ. Salamanca, Campus Miguel de Unamuno, 37007 Salamanca, Spain
These authors contributed equally to this work
...........................................................................................................................................................................................................................
A principal challenge currently facing biologists is how to connect the complete DNA sequence of an organism to its development
and behaviour. Large-scale targeted-deletions have been successful in defining gene functions in the single-celled yeast
Saccharomyces cerevisiae, but comparable analyses have yet to be performed in an animal. Here we describe the use of RNA
interference to inhibit the function of ,86% of the 19,427 predicted genes of C. elegans. We identified mutant phenotypes for 1,722
genes, about two-thirds of which were not previously associated with a phenotype. We find that genes of similar functions are
clustered in distinct, multi-megabase regions of individual chromosomes; genes in these regions tend to share transcriptional
profiles. Our resulting data set and reusable RNAi library of 16,757 bacterial clones will facilitate systematic analyses of the
connections among gene sequence, chromosomal location and gene function in C. elegans.
The ability to inactivate a target gene transiently by RNAi1

  

Source: Ahringe, Julie - Wellcome Trust/Cancer Research UK Gurdon Institute, University of Cambridge
Hahn, Mark E. - Biology Department, Woods Hole Oceanographic Institution

 

Collections: Biology and Medicine; Environmental Sciences and Ecology