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Xenopus Sprouty2 inhibits FGF-mediated gastrulation movements but does not

Summary: Xenopus Sprouty2 inhibits FGF-mediated
gastrulation movements but does not
affect mesoderm induction
and patterning
Stephen L. Nutt,1,3
Kevin S. Dingwell,2,3
Christine E. Holt,2
and Enrique Amaya1,4
Wellcome/CRC Institute, Cambridge CB2 1QR, UK and Department of Zoology, University of Cambridge CB2 3EJ, UK;
Department of Anatomy, University of Cambridge, Cambridge CB2 3DY, UK
Signal transduction through the FGF receptor is essential for the specification of the vertebrate body plan.
Blocking the FGF pathway in early Xenopus embryos inhibits mesoderm induction and results in truncation
of the anterior­posterior axis. The Drosophila gene sprouty encodes an antagonist of FGF signaling, which is
transcriptionally induced by the pathway, but whose molecular functions are poorly characterized. We have
cloned Xenopus sprouty2 and show that it is expressed in a similar pattern to known FGFs and is dependent
on the FGF/Ras/MAPK pathway for its expression. Overexpression of Xsprouty2 in both embryos and explant
assays results in the inhibition of the cell movements of convergent extension. Although blocking
FGF/Ras/MAPK signaling leads to an inhibition of mesodermal gene expression, these markers are unaffected


Source: Amaya, Enrique - Healing Foundation Centre & Developmental Biology, Faculty of Life Sciences, University of Manchester


Collections: Biology and Medicine