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Summary: The effect of swelling and cationic character on gene transfection by
pH-sensitive nanocarriers
Jin-Oh You, Debra T. Auguste*
School of Engineering and Applied Sciences, Harvard University, 29 Oxford Street, Cambridge, MA 02138, USA
a r t i c l e i n f o
Article history:
Received 1 April 2010
Accepted 21 April 2010
Available online 20 May 2010
Keywords:
Nonviral gene delivery
pH-sensitive nanoparticles
DMAEMA
V ATPase
Bafilomycin A1
a b s t r a c t
We synthesized a series of pH-sensitive vehicles, composed of dimethylaminoethyl methacrylate
(DMAEMA) and 2-hydroxyethyl methacrylate (HEMA), to optimize the triggered release of DNA for gene
transfection. The purpose of this study was to assess the role of swelling and cationic character inde-
pendently on transfection; both of which may affect DNA release. Gene transfection was performed by
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