Summary: Cell damage extent due to irradiation with nanosecond laser pulses
under cell culturing medium and dry environment
Francisco G. Pérez-Gutiérrez1
, Gabriel Guillen2
, Rodger Evans3
, Guillermo Aguilar1
1. Department of Mechanical Engineering, University of California, Riverside; 900 University
Ave, Riverside, CA, USA 92521.
2. Instituto de Biotecnología, Universidad Nacional Autónoma de México; Av. Universidad
#2001, Col.Chamilpa C.P. 62210 Cuernavaca, Morelos, México.
3. Departamento de Óptica, Centro de Investigación Científica y de Educación Superior de
Ensenada; Km. 107 Carretera Tijuana - Ensenada C.P. 22860, Ensenada, B.C., México.
Cell mono-layers were irradiated with nanosecond laser pulses under two distinct scenarios: (a) with culturing medium
positioning the beam waist at different stand-off distances and (b) without cell culturing medium, positioning the beam
waist directly on top of the cell mono-layer. Damaged cells were marked with Trypan Blue, a vital cell marker. Three
different zones of damage were identified: (1) a zone of complete cell clearance, surrounded by (2) a ring of dead cells
marked with Trypan Blue and (3) the rest of the cell culture where the cells remain alive and viable. Different
hydrodynamic mechanisms damage cells as it was shown by high speed video for =0 and comparison with time