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The Onset of Homologous Chromosome Pairing during DrosophilamelanogasterEmbryogenesis

Summary: The Onset of Homologous Chromosome Pairing
during DrosophilamelanogasterEmbryogenesis
Yasushi Hiraoka, Abby E Dernburg, Susan J. Parmelee, Mary C. Rykowski, David A. Agard,
and John W. Sedat
DepartmentofBiochemistryand Biophysicsand The HowardHughes MedicalInstitute, UniversityofCalifornia, San Francisco,
Abstract. We have determined the position within the
nucleus of homologous sites of the histone gene clus-
ter in Drosophila melanogaster using in situ hybridiza-
tion and high-resolution, three-dimensional wide field
fluorescence microscopy. A 4.8-kb biotinylated probe
for the histone gene repeat, located approximately
midway along the short arm of chromosome 2, was
hybridized to whole-mount embryos in late syncytial
and early cellular blastoderm stages. Our results show
that the two homologous histone loci are distinct and
separate through all stages of the cell cycle up to
nuclear cycle 13. By dramatic contrast, the two ho-
mologous clusters were found to colocalize with high
frequency during interphase of cycle 14. Concomitant


Source: Agard, David - Department of Biochemistry and Biophysics, University of California at San Francisco


Collections: Biotechnology; Biology and Medicine