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Proc. Nati. Acad. Sci. USA Vol. 83, pp. 4849-4853, July 1986
 

Summary: Proc. Nati. Acad. Sci. USA
Vol. 83, pp. 4849-4853, July 1986
Genetics
Multiple cis-acting elements modulate the translational efficiency of
GCN4 mRNA in yeast
(deletion-insertion mutation/lacZ fusion/GCN2, GCD1 genes/upstream AUG codon)
DIMITRIS TZAMARIAS*t, DESPINA ALEXANDRAKI*, AND GEORGE THIREOS*t
*Institute of Molecular Biology and Biotechnology, Research Center of Crete, P.O. Box 1527, Heraklio, Crete, Greece; and tDepartment of Biology,
University of Crete, Herakhlo, Crete, Greece
Communicated by Fotis C. Kafatos, March 10, 1986
ABSTRACT The expression of the GCN4 gene in yeast is
regulated at the translational level: growth ofyeast cells under
amino acid starvation conditions results in an increase in the
translational efficiency ofGCN4 mRNA. A sequence within the
5' untranslated region ofthis mRNA, which contains four small
open reading frames, acts in cis to suppress translation when
growth occurs in rich media. In this report, we have analyzed
the effects on translation of a series of deletion, insertion, and
substitution mutations in the 5' untranslated region of GCN4
mRNA. This analysis showed that at least two distinct cis

  

Source: Alexandraki, Despina - Institute of Molecular Biology and Biotechnology, Foundation of Research and Technology, Hellas

 

Collections: Biology and Medicine; Biotechnology