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Expression cloning screening of a unique and full-length set of cDNA clones is an efficient method for identifying genes
 

Summary: Expression cloning screening of a unique and full-length set
of cDNA clones is an efficient method for identifying genes
involved in Xenopus neurogenesis
Jana Voigtb
, Jun-An Chenc
, Mike Gilchrista
, Enrique Amayac
, Nancy Papalopulub,*
a
Wellcome Trust/Cancer Research UK Gurdon Institute, Tennis Court Road, Cambridge CB2 1QR, UK
b
Department of Anatomy, University of Cambridge, Downing Site, Cambridge CB2 3DY, UK
c
Department of Zoology, University of Cambridge, Downing Street, Cambridge CB2 3EJ, UK
Received 28 October 2004; accepted 4 November 2004
Abstract
Functional screens, where a large numbers of cDNA clones are assayed for certain biological activity, are a useful tool in elucidating gene
function. In Xenopus, gain of function screens are performed by pool screening, whereby RNA transcribed in vitro from groups of cDNA
clones, ranging from thousands to a hundred, are injected into early embryos. Once an activity is detected in a pool, the active clone is
identified by sib-selection. Such screens are intrinsically biased towards potent genes, whose RNA is active at low quantities. To improve the

  

Source: Amaya, Enrique - Healing Foundation Centre & Developmental Biology, Faculty of Life Sciences, University of Manchester

 

Collections: Biology and Medicine