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Molecular Biology of the Cell Vol. 20, 282295, January 1, 2009

Summary: Molecular Biology of the Cell
Vol. 20, 282295, January 1, 2009
Distinct Apical and Basolateral Membrane Requirements
for Stretch-induced Membrane Traffic at the Apical Surface
of Bladder Umbrella Cells
Weiqun Yu,*
Puneet Khandelwal,* and Gerard Apodaca*
*Laboratory of Epithelial Cell Biology and Renal Electrolyte Division of the Department of Medicine,
Departments of
Bioengineering, and
Cell Biology and Physiology, University of Pittsburgh, Pittsburgh,
PA 15261
Submitted April 30, 2008; Revised October 23, 2008; Accepted October 27, 2008
Monitoring Editor: Keith E. Mostov
Epithelial cells respond to mechanical stimuli by increasing exocytosis, endocytosis, and ion transport, but how these
processes are initiated and coordinated and the mechanotransduction pathways involved are not well understood. We
observed that in response to a dynamic mechanical environment, increased apical membrane tension, but not pressure,
stimulated apical membrane exocytosis and ion transport in bladder umbrella cells. The exocytic response was indepen-
dent of temperature but required the cytoskeleton and the activity of a nonselective cation channel and the epithelial
sodium channel. The subsequent increase in basolateral membrane tension had the opposite effect and triggered the


Source: Apodaca, Gerard - Departments of Medicine & Cell Biology and Physiology, University of Pittsburgh


Collections: Biology and Medicine