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Integration of a splicing regulatory network within the meiotic gene
 

Summary: Integration of a splicing regulatory
network within the meiotic gene
expression program of
Saccharomyces cerevisiae
Elizabeth M. Munding, A. Haller Igel, Lily Shiue, Kristel M. Dorighi, Lisa R. Trevin~ o,
and Manuel Ares Jr.1
Center for Molecular Biology of RNA, Department of Molecular, Cell, and Developmental Biology, Sinsheimer Laboratories,
University of California at Santa Cruz, Santa Cruz, California 95064, USA
Splicing regulatory networks are essential components of eukaryotic gene expression programs, yet little is known
about how they are integrated with transcriptional regulatory networks into coherent gene expression programs.
Here we define the MER1 splicing regulatory network and examine its role in the gene expression program during
meiosis in budding yeast. Mer1p splicing factor promotes splicing of just four pre-mRNAs. All four Mer1p-
responsive genes also require Nam8p for splicing activation by Mer1p; however, other genes require Nam8p but
not Mer1p, exposing an overlapping meiotic splicing network controlled by Nam8p. MER1 mRNA and three of the
four Mer1p substrate pre-mRNAs are induced by the transcriptional regulator Ume6p. This unusual arrangement
delays expression of Mer1p-responsive genes relative to other genes under Ume6p control. Products of Mer1p-
responsive genes are required for initiating and completing recombination and for activation of Ndt80p, the
activator of the transcriptional network required for subsequent steps in the program. Thus, the MER1 splicing
regulatory network mediates the dependent relationship between the UME6 and NDT80 transcriptional
regulatory networks in the meiotic gene expression program. This study reveals how splicing regulatory networks

  

Source: Ares Jr., Manny - Department of Molecular, Cell, and Developmental Biology, University of California at Santa Cruz

 

Collections: Biology and Medicine