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Proc. Natl. Acad. Sci. USA Vol. 77, No. 1, pp. 191-195, January 1980
 

Summary: Proc. Natl. Acad. Sci. USA
Vol. 77, No. 1, pp. 191-195, January 1980
Biochemistry
Interspecies homology of nitrogenase genes
(recombinant DNA/restriction endonuclease mapping/DNA-DNA hybridization)
GARY B. RUVKUN AND FREDERICK M. AUSUBEL
Cellular and Developmental Biology Group, Department of Biology, Biological Laboratories, Harvard University, Cambridge, Massachusetts 02138
Communicated by Lawrence Bogorad, September 27, 1979
ABSTRACT Cloned nitrogen fixation (nifl) genes from
Klebsiella pneumoniae hybridize to DNA from 19 out of 19
widely divergent nitrogen-fixing bacterial strains but do not
hybridize to DNA from 10 different non-nitrogen-fixing species.
K pneumoniae nifDNA fragments that hybridize to DNA from
other species contain part of the three structural genes that code
for nitrogenase polypeptides. We have utilized this homology
to clone an EcoRI restriction endonuclease fragment from
Rhizobium meliloti that hybridizes to the K. pneumoniae nif
structural genes. Some of the species whose DNA hybridizes
with K. pneumoniae nif DNA have been postulated to have
diverged from K pneumoniae 3 X 109 years ago. Nitrogenase

  

Source: Ausubel, Frederick M. - Department of Genetics, Harvard University

 

Collections: Biology and Medicine