Home

About

Advanced Search

Browse by Discipline

Scientific Societies

E-print Alerts

Add E-prints

E-print Network
FAQHELPSITE MAPCONTACT US


  Advanced Search  

 
Proc. Natl. Acad. Sci. USA Vol. 74, No. 7, pp. 2963-2967, July 1977
 

Summary: Proc. Natl. Acad. Sci. USA
Vol. 74, No. 7, pp. 2963-2967, July 1977
Genetics
Recombinant plasmid that carries part of the nitrogen fixation (nif)
gene cluster of Klebsiella pneumoniae
(cloning/distamycin A/restriction enzymes/phage P1 cotransduction)
FRANK C. CANNON*, GERARD E. RIEDELO, AND FREDERICK M. AUSUBELtt
* Agricultural Research Council Unit of Nitrogen Fixation, University of Sussex, Falmer, Brighton, BN1 9QJ, United Kingdom; and t Department of Biology,
Harvard University, Cambridge, Massachusetts 02138
Communicated by Lawrence Bogorad, April25, 1977
ABSTRACT We have cloned fragments of the Klebsiella
pneumoniae genome that carry part of the his o ron and part
of the nitrogen fixation (nif) gene cluster on the amplifiable
plasmid pMB9. One particular plasmid, pCRA37, complements
mutations in the hisD, nifE, and nifF loci. The physical map
of pCRA37 as determined by restriction enzyme analysis cor-
relates with the genetic map of the his-nifregion as determined
previously by phage P1-mediated cotransductional analysis.
The vast majority of nitrogen atoms in living organisms are
derived from theatmosphereby certain species ofbacteria and

  

Source: Ausubel, Frederick M. - Department of Genetics, Harvard University

 

Collections: Biology and Medicine