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Proc. Nati. Acad. Sci. USA Vol. 82, pp. 5841-5845, September 1985
 

Summary: Proc. Nati. Acad. Sci. USA
Vol. 82, pp. 5841-5845, September 1985
Genetics
Conservation of nodulation genes between Rhizobium meliloti and a
slow-growing Rhizobium strain that nodulates a nonlegume host
(Parasponia Rhizobium/symbiosis/interspecies complementation)
DEBORAH J. MARVEL*t, GRETCHEN KULDAUt, ANN HIRSCHt, ERIC RICHARDS*§, JOHN G. TORREYt,
AND FREDERICK M. AUSUBEL*§
§Department of Genetics, Harvard Medical School, and *Department of Molecular Biology, Massachusetts General Hospital, Boston, MA 02114; tDepartment
of Biology, Wellesley College, Wellesley, MA 02532; and tDepartment of Organismal and Evolutionary Biology, Harvard University, Cambridge, MA 02138
Contributed by John G. Torrey, May 6, 1985
ABSTRACT Parasponia, a woody member ofthe elm fam-
ily, is the only nonlegume genus whose members are known to
form an effective nitrogen-fixing symbiosis with a Rhizobium
species. The bacterial strain RP501 is a slow-growing strain of
Rhizobium isolated fromParasponia nodules. Strain RP501 also
nodulates the legumes siratro (Macroptilium atropurpureum)
and cowpea (Vigna unguiculata). Using a cosmid clone bank of
RP501 DNA, we isolated a 13.4-kilobase (kb) EcoRI fragment
that complemented insertion and point mutations in three

  

Source: Ausubel, Frederick M. - Department of Genetics, Harvard University

 

Collections: Biology and Medicine