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MOLECULAR AND CELLULAR BIOLOGY, July 2004, p. 59535966 Vol. 24, No. 13 0270-7306/04/$08.00 0 DOI: 10.1128/MCB.24.13.59535966.2004
 

Summary: MOLECULAR AND CELLULAR BIOLOGY, July 2004, p. 59535966 Vol. 24, No. 13
0270-7306/04/$08.00 0 DOI: 10.1128/MCB.24.13.59535966.2004
Copyright 2004, American Society for Microbiology. All Rights Reserved.
CtBP Contributes Quantitatively to Knirps Repression Activity in an
NAD Binding-Dependent Manner
Montserrat Sutrias-Grau1
and David N. Arnosti1,2
*
Department of Biochemistry and Molecular Biology1
and Genetics Program,2
Michigan State
University, East Lansing, Michigan 48824-1319
Received 11 November 2003 /Returned for modification 26 January 2004 /Accepted 29 March 2004
Transcriptional repressors often employ multiple activities, but the molecular mechanisms and physiolog-
ical relevance of this functional diversity remain obscure. The Drosophila melanogaster Knirps repressor uses
CtBP corepressor-dependent and -independent pathways. To separately analyze the components of Knirps
repression activity, we elucidated the specific repression properties of CtBP and of Knirps subdomains. Like
Knirps, CtBP represses adjacent transcriptional activators; but unlike Knirps, CtBP is unable to repress basal
promoter elements. We determined that the ability of CtBP to recapitulate only a subset of Knirps activities
is due to a quantitative, rather than qualitative, deficiency in repression activity. The CtBP-dependent portion

  

Source: Arnosti, David N. - Department of Biochemistry and Molecular Biology, Michigan State University

 

Collections: Biology and Medicine