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Role of Toxoplasma gondii Myosin A in Powering Parasite

Summary: Role of Toxoplasma gondii
Myosin A in Powering Parasite
Gliding and Host Cell Invasion
Markus Meissner,1
Dirk Schlušter,2
Dominique Soldati1
Obligate intracellular apicomplexan parasites rely on gliding motion powered
by their actomyosin system to disperse throughout tissues and to penetrate
host cells. Toxoplasma gondii myosin A has been implicated in this process, but
direct proof has been lacking. We designed a genetic screen to generate a
tetracycline-inducible transactivator system in T. gondii. The MyoA gene was
disrupted in the presence of a second regulatable copy of MyoA. Conditional
removal of this myosin caused severe impairment in host cell invasion and
parasite spreading in cultured cells, and unambiguously established the patho-
genic function of this motor in an animal model.
Among the many vital functions of an obli-
gate intracellular parasite, host cell invasion
is a prerequisite for survival and replication,
and this process is dependent on the ability of


Source: Arnold, Jonathan - Nanoscale Science and Engineering Center & Department of Genetics, University of Georgia


Collections: Biotechnology