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Patterning Proteins and Cells Using Two-Dimensional Arrays of Colloids
 

Summary: Patterning Proteins and Cells Using Two-Dimensional
Arrays of Colloids
Nathaniel J. Gleason, Christopher J. Nodes, Eileen M. Higham,
Nedra Guckert, Ilhan A. Aksay, Jean E. Schwarzbauer, and
Jeffrey D. Carbeck*,
Department of Chemical Engineering, and Department of Molecular Biology,
Princeton University, Princeton, New Jersey 08544
Received September 11, 2002. In Final Form: November 18, 2002
Asimplemethodisdescribedforcontrollingtheorganizationofproteinsonsurfacesusingtwo-dimensional
arrays of micron-sized colloidal particles. Suspensions of colloids functionalized with proteins are deposited
onto coverslips coated with gold using a combination of gravitational settling and applied electrical fields.
Varying settling time and particle concentration controls the density of particles on the substrate. Surface
coverage ranged from an essentially continuous coating of protein on close-packed arrays to domains of
protein separated by distances as large as 16 Ám. Colloidal particle arrays were also patterned into 500
Ám islands on substrates using elastomeric lift-off membranes. The applicability of this approach to the
promotion of fibroblast cell adhesion and spreading was demonstrated using particles coated with the cell
adhesion protein fibronectin. Behavior of adherent cells varied with particle density. This method provides
a general strategy for controlling the organization of functional proteins at surfaces on three length scales:
the size of individual colloidal particles, the spacing between particles, and the organization of particles
in patterned arrays.

  

Source: Aksay, Ilhan A. - Department of Chemical Engineering, Princeton University

 

Collections: Materials Science