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Gfi1 and Gfi1b act equivalently in haematopoiesis, but have distinct, non-overlapping functions in
 

Summary: Gfi1 and Gfi1b act equivalently in haematopoiesis,
but have distinct, non-overlapping functions in
inner ear development
Katharina Fiolka1, Ronna Hertzano2, Lothar Vassen1, Hui Zeng1, Orit Hermesh2, Karen B. Avraham2,
Ulrich Du¨hrsen3 & Tarik Mo¨ro¨y1+
1Institut fu¨r Zellbiologie (Tumorforschung), IFZ, Universita¨tsklinikum Essen, Essen, Germany, 2Department of Human Genetics and
Molecular Medicine, Sackler School of Medicine, Tel Aviv University, Tel Aviv, Israel, and 3Klinik fu¨r Ha¨matologie,
Universita¨tsklinikum Essen, Essen, Germany
Gfi1 is a transcriptional repressor essential for haematopoiesis
and inner ear development. It shares with its paralogue Gfi1b an
amino-terminal SNAG repressor domain and six carboxy-terminal
zinc-finger motifs, but differs from Gfi1b in sequences separating
these domains. Here, we describe two knock-in mouse models,
in which the N-terminal SNAG repressor domain was mutated or
in which the Gfi1 coding region was replaced by Gfi1b. Mouse
mutants without an intact SNAG domain show the full phenotype
of Gfi1 null mice. However, Gfi1:Gfi1b knock-in mice show
almost normal pre-T-cell and neutrophil development, but lack
properly formed inner ear hair cells. Hence, our findings show
that an intact SNAG domain is essential for all functions of Gfi1

  

Source: Avraham, Karen - Department of Human Genetics and Molecular Medicine, Tel Aviv University

 

Collections: Biology and Medicine