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Northern Blots (based on protocol from Mitch Smith Lab)
 

Summary: Auble Lab
Northern Blots
(based on protocol from Mitch Smith Lab)
Use gel apparatus designated for RNA. Wipe apparatus with "RNase Away".
1% Agarose gel (100ml)
73 ml sterile water
1 g agarose
Boil in microwave and cool a little
Add 10 ml 10X MOPS and 17 ml 37% Formaldehyde
Pour.
10X MOPS
0.2 M MOPS 23.1 g/500ml
50 mM Sodium Acetate 8.3 ml of 3M/500 ml
10mM EDTA 10ml of 0.5M/500ml
adjust pH 7.0
Sample preparation and electrophoresis
Add 20 g each RNA to eppendorf tube, dry samples in speed-vac. Add 17 l Sample
Buffer, heat samples at 65 C for 10 min, add 3 l 10X dye, and then load gel and run in
1X MOPS
Run gel 1-2 hrs at 90 V

  

Source: Auble, David - Department of Biochemistry and Molecular Genetics, University of Virginia

 

Collections: Biology and Medicine