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Copyright 0 1997by the Genetics Society of America Phytoalexin-DeficientMutants of Arabidopsis Reveal ThatPAD4 Encodes
 

Summary: Copyright 0 1997by the Genetics Society of America
Phytoalexin-DeficientMutants of Arabidopsis Reveal ThatPAD4 Encodes
a Regulatory Factor and That Four PAD Genes Contribute
to Downy Mildew Resistance
Jane Glazebrook,*.t Michael Zook,I Figen Mert, Isabelle Kagan,' Elizabeth E. Rogers,t Ian R. Crute,
Eric B. Holub, Raymond Hammerschmidt' and Frederick M. Ausubelt
*Centerfor Agricultural Biotechnology, University of Maryland Biotechnology Institute, College Park, Maryland 20742, tDepartment of
Genetics, Haroard Medical School and Department of Molecular Biology, Massachusetts General Hospital, Boston, Massachusetts
021 14,$Department of Botany and Plant Pathology, Michigan State University, East Lansing, Michigan 48824, and $Plant
Pathology and Weed Science Department, Horticulture Research International, Wellesbourne, Wanuick CV35 9EF, United Kingdom
Manuscript received October 18, 1996
Accepted for publication January 7, 1997
ABSTRACT
We are working to determine the role of the Arabidopsis phytoalexin, camalexin, in protecting the
plant from pathogen attack by isolating phytoalexindeficient (pad) mutants in the accession Columbia
(Col-0) and examining their response to pathogens. Mutations in PADI, PAD2, and PAD4 caused en-
hanced susceptibility tothe bacterial pathogen Pseudomonas syringae pv. maculicola strain ES4326
(PsmES4326), while mutations in PAD3 or PAD5 did not. Camalexin was not detected in any of the
double mutants padl-l pad2-I, padl-l pad3-1 or pad2-1 pad3-I. Growth of PsmES4326 in padl-1 pad2-1
was greaterthanthatin padl-1 or pad2-1 plants, while growth in padl-1pad3-l and pad2-1pad3-1

  

Source: Ausubel, Frederick M. - Department of Genetics, Harvard University

 

Collections: Biology and Medicine