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Summary: Transgenic Frog Embryos 393
393
From: Methods in Molecular Biology, Vol. 97: Molecular Embryology: Methods and Protocols
Edited by: P. T. Sharpe and I. Mason © Humana Press Inc., Totowa, NJ
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A Method for Generating Transgenic Frog Embryos
Enrique Amaya and Kristen L. Kroll
1. Introduction
1.1. Summary
The early amphibian embryo has been widely used as a model organism for
studying early vertebrate development. This chapter describes in detail a new
and very efficient method for generating transgenic Xenopus embryos. At the
end of the chapter, a new method for fertilizing in vitro matured oocytes is also
introduced.
1.2. Background
The elucidation of the molecular basis of pattern formation and differentia-
tion in frog embryos has been hindered by the lack of a system for temporal
and tissue-specific expression of wild type and mutant forms of developmen-
tally important genes. RNA injection, the most common transient expression
method in Xenopus, has been effectively used to study maternally expressed
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