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Yeast Genomic DNA Prep Sterile distilled water
 

Summary: Auble Lab
Yeast Genomic DNA Prep
Reagents:
Sterile distilled water
-mercaptoethanol
Sorbitol Buffer: 1 M sorbitol, 0.1 M sodium citrate (pH 5.8), 0.01 M EDTA (pH 8.0)
Saline-EDTA Buffer: 0.15 M NaCl, 0.1 M EDTA (pH 8.0), 0.1% SDS
Lyticase ( or T100 Zymolase)
95% Ethanol
70% Ethanol
10 mg/ml Rnase
phenol
TE
Protocol:
1. Grow 25 ml of yeast cells in YPD to 5x107
cells/ml.
2. Spin down cells in 50-ml conical tube at 3,000 rpms for 5 minutes.
3. Resuspend in 10 ml of sterile distilled (SD) water, then spin for 5 minutes
at 3,000 rpms and decant off water.
4. Resuspend in 5 ml of SD water and add 100 Ál of -mercaptoethanol.

  

Source: Auble, David - Department of Biochemistry and Molecular Genetics, University of Virginia

 

Collections: Biology and Medicine