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Gene,69 (1988)231-244 Molecular analysis of the gene encoding a-lytic protease: evidence for a preproenzyme
 

Summary: Gene,69 (1988)231-244
Elsevier
231
GEN 02559
Molecular analysis of the gene encoding a-lytic protease: evidence for a preproenzyme
(Recombinant DNA; extracellular serine protease; Lysobacter enzymogenes; cloning in E. coli; S 1 mapping;
sequencing high GC DNA; subtilisin)
J.L. Silen, C.N. McGrath *, K.R. Smith and D.A. Agard
Department ofBiochemistry and Biophysicsand The Howard Hughes Medical Institute,Universityof Californiaat San Francisco,
San Francisco, CA 94143-0448 (U.S.A.) Tel. (415)476-5143
Received 22 February 1988
Accepted 12 May 1988
Received by publisher 6 June 1988
SUMMARY
A 1.7-kb EcoRI fragment containing the structural gene for a-lytic protease has been cloned from Lysobacter
enzymogenes 495 chromosomal DNA: the first example of a gene cloned from this organism. The protein
sequence deduced from the nucleotide sequence encoding this serine protease matches the published amino
acid sequence [Olson et al., Nature 228 (1970) 438-4421 precisely. Sequence analysis and S 1 mapping indicate
that, like subtilisin [e.g., Wells et al., Nucleic Acids Res. 11 (1983) 791 l-79251 a-lytic protease is synthesized
as a pre-pro protein (41 kDa) that is subsequently processed to its mature extracellular form (20 kDa). This

  

Source: Agard, David - Department of Biochemistry and Biophysics, University of California at San Francisco

 

Collections: Biotechnology; Biology and Medicine