Summary: Mot1 activates and represses transcription by direct,
ATPase-dependent mechanisms
Arindam Dasgupta*
, Russell P. Darst*
, Karla J. Martin
, Cynthia A. Afshari
, and David T. Auble*§
*Department of Biochemistry and Molecular Genetics, University of Virginia Health System, 1300 Jefferson Park Avenue, Room 6213, Charlottesville, VA
22908-0733; and National Institute of Environmental Health Sciences Microarray Center, 111 Alexander Drive, Building 101, Room D226,
PO Box 12233, MD2­04, Research Triangle Park, NC 27709
Edited by Robert G. Roeder, The Rockefeller University, New York, NY, and approved January 4, 2002 (received for review July 30, 2001)
Mot1 is an essential yeast Snf2 Swi2-related ATPase that exerts
both positive and negative effects on gene expression. In vitro,
Mot1 can disrupt TATA-binding protein­DNA complexes in an
ATP-dependent reaction. This activity can explain Mot1-mediated
transcriptional repression, but how Mot1 activates transcription is
unknown. We demonstrate that, remarkably, Mot1 is localized in
vivo to promoters for both Mot1-repressed and Mot1-activated
genes. Moreover, Mot1 ATPase activity is required for both acti-
vation and repression of gene activity. These findings suggest a

Source: Auble, David - Department of Biochemistry and Molecular Genetics, University of Virginia

Collections: Biology and Medicine