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Z .Biophysical Chemistry 75 1998 213 227 Oligomerization and phase separation in globular protein
 

Summary: Z .Biophysical Chemistry 75 1998 213 227
Oligomerization and phase separation in globular protein
solutions
Neer Asheriea
, Jayanti Pandea
, Aleksey Lomakina
, Olutayo Oguna
,
Stacy R.A. Hansonb
, Jean B. Smithb
, George B. Benedeka,U
a
Department of Physics and Center for Materials Science and Engineering, Rm 13-2005, Massachusetts Institute of
Technology, 77 Massachusetts Ašenue, Cambridge, MA 02139-4307, USA
b
Department of Chemistry, Unišersity of Nebraska, Lincoln, NE 68588-0304, USA
Received 31 August 1998; accepted 24 September 1998
Abstract
We have chemically crosslinked a globular protein, -crystallin, to produce a system of well-defined oligomers:IIIb
monomers, dimers, trimers and a mixture of higher n-mers. Gel electrophoresis, size exclusion chromatography,

  

Source: Asherie, Neer - Departments of Physics & Biology, Yeshiva University
Benedek, George B. - Department of Physics, Massachusetts Institute of Technology (MIT)

 

Collections: Biology and Medicine; Physics