Summary: derived craniofacial and skeletal elements, our work complements
early grafting experiments that demonstrated the influence of the
mesoderm on epidermal differentiation9
. A
Methods
Generation of CK14­Ikka transgenic mice
A HindIII­NotI fragment encoding human Ikka was subcloned into the SnaBI site of
pBS-R3, which contains the human CK14 promoter. The SalI­NotI fragment containing a
CK14­Ikka poly(A) cassette was isolated, purified and microinjected into male pro-nuclei of
fertilized C57BL/6 oocytes. The CK14­Ikka(KM) construct was similarly generated except
that the expression cassette was injected into oocytes isolated from an Ikkaþ/2
intercross.
Transgenic founders were identified by PCR analysis and confirmed by immunoblotting.
Subsequently, two different lines of transgenic founders were crossed with Ikkaþ/2
mice.
Ikkaþ/2
CK14­Ikka mice were crossbred to generate rescued knockout mice. Ikka2/2
CK14­Ikka(KM) mice were identified by genotyping of tissues derived from neonate mice.
Immunoblotting and immunohistochemistry
Protein lysates were prepared from different tissues of Ikka2/2

Source: Akabas, Myles - Department of Physiology and Biophysics, Albert Einstein College of Medicine, Yeshiva University
Murry, Chuck - Center for Cardiovascular Biology & Department of Bioengineering, University of Washington at Seattle

Collections: Biology and Medicine