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May 15, 2003 / Vol. 28, No. 10 / OPTICS LETTERS 801 Phase retrieval for high-numerical-aperture optical systems
 

Summary: May 15, 2003 / Vol. 28, No. 10 / OPTICS LETTERS 801
Phase retrieval for high-numerical-aperture optical systems
Bridget M. Hanser
Graduate Group in Biophysics, University of California, San Francisco, San Francisco, California 94143
Mats G. L. Gustafsson, David A. Agard, and John W. Sedat
Department of Biochemistry and Biophysics, University of California, San Francisco, San Francisco, California 94143
Received November 15, 2002
We describe a phase retrieval approach for intensity point-spread functions of high-numerical-aperture optical
systems such as light microscopes. The method calculates a generalized pupil function defined on a spherical
shell, using measured images at several defocus levels. The resultant pupil functions reproduce measured
point-source images significantly better than does an ideal imaging model. Availability of pupil function
information will facilitate new approaches to aberration correction in such systems. 2003 Optical Society
of America
OCIS codes: 100.5670, 070.2580, 180.0180, 100.0100.
Phase retrieval is a technique for estimating the
wave-front properties of an imaging system from
one or more intensity images, typically of a point
source.1
The retrieved wave-front information takes
the form of a complex pupil function, which permits a

  

Source: Agard, David - Department of Biochemistry and Biophysics, University of California at San Francisco

 

Collections: Biotechnology; Biology and Medicine