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Position estimation of fluorescent probes in a confocal microscope Sean B. Andersson
 

Summary: Position estimation of fluorescent probes in a confocal microscope
Sean B. Andersson
Abstract-- Measurements of fluorescence intensity from an
isolated fluorescing probe in a confocal microscope can be
viewed as range-only measurements of the position of that
probe. We present an analytical algorithm for determining its
position from a collection of measurements taken at different
locations in the plane. This algorithm, fluoroBancroft, is in-
spired by the Bancroft algorithm in GPS. We investigate the
performance of the algorithm through simulation and compare
it to the standard technique of fitting the data to a Gaussian
profile. Our results indicate that the fluoroBancroft algorithm
is more accurate than the fitting procedure when only a few
measurements are used. Moreover the technique is typically
two orders of magnitude faster in terms of computation time.
These preliminary results indicate the fluoroBancroft algorithm
holds promise for use in a real-time closed-loop controller to
track the motion of single fluorescent probes.
I. INTRODUCTION
Single particle tracking in fluorescence microscopy has be-

  

Source: Andersson, Sean B. - Department of Aerospace and Mechanical Engineering, Boston University

 

Collections: Engineering