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AUTOMATIC QUANTIFICATION OF IN VITRO ENDOTHELIAL CELL NETWORKS USING MATHEMATICAL MORPHOLOGY
 

Summary: AUTOMATIC QUANTIFICATION OF IN VITRO ENDOTHELIAL CELL
NETWORKS USING MATHEMATICAL MORPHOLOGY
Jes´us Angulo Sabine Matou
Centre de Morphologie Math´ematique, Department of Biological Sciences,
Ecole des Mines de Paris, France The Manchester Metropolitan University, UK
angulo@cmm.ensmp.fr ;http://cmm.ensmp.fr/angulo S.Matou@mmu.ac.uk
ABSTRACT
Experiments of in vitro angiogenesis are important tools
for studying both the mechanisms of formation of new
blood vessels and the potential development of therapeutic
strategies to modulate neovascularisation (screening of new
pharmacological molecules). One of the most frequently
used angiogenesis assays is the culture of endothelial cells
on a reconstituted basement membrane named Matrigel,
since the cells constitute a capillary-like network which can
be quantified by image analysis. In this paper, a global, ro-
bust and fully automated methodology is proposed to seg-
ment and quantify in vitro endothelial cell networks from
greyscale images using operators from mathematical mor-
phology. Experimental results allow us to draw a discus-

  

Source: Angulo,Jesús - Centre de Morphologie Mathématique, Ecole des Mines de Paris

 

Collections: Computer Technologies and Information Sciences